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Flow cytometry analysis is an analytical method that simultaneously measures the physical and chemical properties of cells at specific wavelengths by transmitting light through cells in a fluid state.
This analytical method is primarily used to identify biomarkers for monitoring immune responses and cellular changes in oncology research and new drug development. It also plays an essential role across the biotechnology industry, including in the identification, purity, and potency testing of advanced biopharmaceuticals.
Real-Time Polymerase Chain Reaction (RT-PCR) is a molecular biology analytical method that replicates and amplifies specific DNA and quantifies its amount in real time.
This method, with its high sensitivity and specificity, is used to measure nucleic acids in various biological samples and to compare and quantify gene expression.
Enzyme-Linked Immunospot (ELISPOT) is an analytical method that can analyze target substances, such as cytokines, secreted in response to specific antigen stimulation at the single-cell level.
Even trace amounts of substances can be precisely measured.
The Enzyme-Linked Immunosorbent Assay (ELISA) is an enzyme-linked immunosorbent assay that quantifies target substances through antigen-antibody binding and the enzymatic reaction that follows.
It typically measures the amount of target antigen using capture antibodies adsorbed to a 96-well plate.